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rabbit polyclonal antibodies against cul3  (Bethyl)


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    Structured Review

    Bethyl rabbit polyclonal antibodies against cul3
    (A) MA5.8ζ IL-2 secretion at 12 h normalized to the number of live cells measured by annexin V and propidium iodide staining and (B) transcription at 12 h with 4 µg/mL α-CD3 and the indicated concentrations of OPT (n = 3). (C–G) IL-2 secretion by MA5.8ζ cells, cells expressing control shRNA, and cells expressing an shRNA for (C) CUL1, (D) CUL2, (E) <t>CUL3,</t> (F) CUL4a/b and (G) CUL5 after 48 h of stimulation with indicated concentrations of α-CD3. Data are averaged from two independent experiments, where each experiment examined three independently created lines for each shRNA. (H) IL-2 secretion at 48 h by MA5.8ζ cells and cells expressing either control shRNA, CUL2 shRNA or <t>CUL3</t> shRNA with 1 µg/mL α-CD3 and 200 nM MLN4924. Data are averaged as in (C–G). Values represent mean ± s.e.m.; * P <0.05, ** P <0.01, *** P <0.001.
    Rabbit Polyclonal Antibodies Against Cul3, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibodies against cul3/product/Bethyl
    Average 93 stars, based on 73 article reviews
    rabbit polyclonal antibodies against cul3 - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "The Discovery of a Reciprocal Relationship between Tyrosine-Kinase Signaling and Cullin Neddylation"

    Article Title: The Discovery of a Reciprocal Relationship between Tyrosine-Kinase Signaling and Cullin Neddylation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0075200

    (A) MA5.8ζ IL-2 secretion at 12 h normalized to the number of live cells measured by annexin V and propidium iodide staining and (B) transcription at 12 h with 4 µg/mL α-CD3 and the indicated concentrations of OPT (n = 3). (C–G) IL-2 secretion by MA5.8ζ cells, cells expressing control shRNA, and cells expressing an shRNA for (C) CUL1, (D) CUL2, (E) CUL3, (F) CUL4a/b and (G) CUL5 after 48 h of stimulation with indicated concentrations of α-CD3. Data are averaged from two independent experiments, where each experiment examined three independently created lines for each shRNA. (H) IL-2 secretion at 48 h by MA5.8ζ cells and cells expressing either control shRNA, CUL2 shRNA or CUL3 shRNA with 1 µg/mL α-CD3 and 200 nM MLN4924. Data are averaged as in (C–G). Values represent mean ± s.e.m.; * P <0.05, ** P <0.01, *** P <0.001.
    Figure Legend Snippet: (A) MA5.8ζ IL-2 secretion at 12 h normalized to the number of live cells measured by annexin V and propidium iodide staining and (B) transcription at 12 h with 4 µg/mL α-CD3 and the indicated concentrations of OPT (n = 3). (C–G) IL-2 secretion by MA5.8ζ cells, cells expressing control shRNA, and cells expressing an shRNA for (C) CUL1, (D) CUL2, (E) CUL3, (F) CUL4a/b and (G) CUL5 after 48 h of stimulation with indicated concentrations of α-CD3. Data are averaged from two independent experiments, where each experiment examined three independently created lines for each shRNA. (H) IL-2 secretion at 48 h by MA5.8ζ cells and cells expressing either control shRNA, CUL2 shRNA or CUL3 shRNA with 1 µg/mL α-CD3 and 200 nM MLN4924. Data are averaged as in (C–G). Values represent mean ± s.e.m.; * P <0.05, ** P <0.01, *** P <0.001.

    Techniques Used: Staining, Expressing, Control, shRNA



    Similar Products

    rabbit polyclonal antibodies against cul3  (Bethyl)
    93
    Bethyl rabbit polyclonal antibodies against cul3
    (A) MA5.8ζ IL-2 secretion at 12 h normalized to the number of live cells measured by annexin V and propidium iodide staining and (B) transcription at 12 h with 4 µg/mL α-CD3 and the indicated concentrations of OPT (n = 3). (C–G) IL-2 secretion by MA5.8ζ cells, cells expressing control shRNA, and cells expressing an shRNA for (C) CUL1, (D) CUL2, (E) <t>CUL3,</t> (F) CUL4a/b and (G) CUL5 after 48 h of stimulation with indicated concentrations of α-CD3. Data are averaged from two independent experiments, where each experiment examined three independently created lines for each shRNA. (H) IL-2 secretion at 48 h by MA5.8ζ cells and cells expressing either control shRNA, CUL2 shRNA or <t>CUL3</t> shRNA with 1 µg/mL α-CD3 and 200 nM MLN4924. Data are averaged as in (C–G). Values represent mean ± s.e.m.; * P <0.05, ** P <0.01, *** P <0.001.
    Rabbit Polyclonal Antibodies Against Cul3, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibodies against cul3/product/Bethyl
    Average 93 stars, based on 1 article reviews
    rabbit polyclonal antibodies against cul3 - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) MA5.8ζ IL-2 secretion at 12 h normalized to the number of live cells measured by annexin V and propidium iodide staining and (B) transcription at 12 h with 4 µg/mL α-CD3 and the indicated concentrations of OPT (n = 3). (C–G) IL-2 secretion by MA5.8ζ cells, cells expressing control shRNA, and cells expressing an shRNA for (C) CUL1, (D) CUL2, (E) CUL3, (F) CUL4a/b and (G) CUL5 after 48 h of stimulation with indicated concentrations of α-CD3. Data are averaged from two independent experiments, where each experiment examined three independently created lines for each shRNA. (H) IL-2 secretion at 48 h by MA5.8ζ cells and cells expressing either control shRNA, CUL2 shRNA or CUL3 shRNA with 1 µg/mL α-CD3 and 200 nM MLN4924. Data are averaged as in (C–G). Values represent mean ± s.e.m.; * P <0.05, ** P <0.01, *** P <0.001.

    Journal: PLoS ONE

    Article Title: The Discovery of a Reciprocal Relationship between Tyrosine-Kinase Signaling and Cullin Neddylation

    doi: 10.1371/journal.pone.0075200

    Figure Lengend Snippet: (A) MA5.8ζ IL-2 secretion at 12 h normalized to the number of live cells measured by annexin V and propidium iodide staining and (B) transcription at 12 h with 4 µg/mL α-CD3 and the indicated concentrations of OPT (n = 3). (C–G) IL-2 secretion by MA5.8ζ cells, cells expressing control shRNA, and cells expressing an shRNA for (C) CUL1, (D) CUL2, (E) CUL3, (F) CUL4a/b and (G) CUL5 after 48 h of stimulation with indicated concentrations of α-CD3. Data are averaged from two independent experiments, where each experiment examined three independently created lines for each shRNA. (H) IL-2 secretion at 48 h by MA5.8ζ cells and cells expressing either control shRNA, CUL2 shRNA or CUL3 shRNA with 1 µg/mL α-CD3 and 200 nM MLN4924. Data are averaged as in (C–G). Values represent mean ± s.e.m.; * P <0.05, ** P <0.01, *** P <0.001.

    Article Snippet: Rabbit polyclonal antibodies against CUL3 (A301-109A) and CUL5 (A302-173A) were purchased from Bethyl Laboratories, Inc.

    Techniques: Staining, Expressing, Control, shRNA